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1.
Braz. j. biol ; 76(1): 55-58, Feb. 2016. graf
Article in English | LILACS | ID: lil-774512

ABSTRACT

Abstract Paca (Cuniculus paca Linnaeus, 1766) is the second largest rodent found in Brazil. The quality of the meat and a long tradition of hunting have contributed to the decline of the natural populations of this species. Hunting of paca is strictly prohibited in Brazil, but in spite of this restriction, no forensic tools are available for the identification of the meat. We describe an efficient method, based on single nucleotide polymorphisms of the cytochrome b gene, that can be used to differentiate biological material derived from paca from those of domestic species commonly used as sources of meat. The identification of the presence of C. paca in the samples was 100% reliable.


Resumo Paca (Cuniculus paca Linnaeus, 1766) é o segundo maior roedor brasileiro. A qualidade da carne e a forte tradição da caça de subsistência são fatores que contribuem significativamente para o declínio das populações. Apesar da proibição a caça no Brasil, no momento ainda não há ferramentas disponíveis para identificar a carne e seus produtos como prova forense. Neste trabalho propomos um método eficaz de identificação, baseado em polimorfismos de único nucleotídeo no gene Citocromo b, objetivando diferenciar material biológico de paca das espécies domésticas comumente utilizadas como alimento no Brasil. A identificação das amostras de paca foram possíveis em 100% das amostras analisadas.


Subject(s)
Animals , Conservation of Natural Resources/methods , Cuniculidae/genetics , Cytochromes b/analysis , Meat/analysis , Amino Acid Sequence , Brazil , Cuniculidae/classification , Meat/classification , Sequence Alignment
2.
Braz. j. biol ; 75(2): l4341-434, 05/2015. graf
Article in English | LILACS, VETINDEX | ID: biblio-1468212

ABSTRACT

Analyses of 16S rDNA genes were used to identify the microbiota isolated from the mucus of the zoanthid Palythoa caribaeorum at Porto de Galinhas on the coast of Pernambuco State, Brazil. This study is important as the first report of this association, because of the potential biotechnological applications of the bacterium Alcanivorax dieselolei, and as evidence for the presence of a hydrocarbon degrading bacterium in a reef ecosystem such as Porto de Galinhas.


Análises dos genes 16S rDNA foram empregadas para identificar a microbiota isolada do muco do zoantídeo Palythoa caribaeorum de Porto de Galinhas, litoral do estado de Pernambuco, Brasil. Este estudo é importante pelo ineditismo dessa associação, pelas relevantes aplicações biotecnológicas da bactéria Alcanivorax dieselolei e pela indicação da presença de uma bactéria degradadora de hidrocarbonetos em um ecossistema recifal como o de Porto de Galinhas.


Subject(s)
Animals , Alcanivoraceae/genetics , Anthozoa/microbiology , Mucus/microbiology , Alcanivoraceae/physiology , DNA, Bacterial/genetics , /genetics , Polymerase Chain Reaction
3.
Genet. mol. res. (Online) ; 5(4): 564-568, 2006. tab, ilus
Article in English | LILACS | ID: lil-482097

ABSTRACT

We examined the variation in mitochondrial DNA by sequencing the D-loop region in wild and domestic (large-white breed) pigs, in hybrids between domestic and wild pigs, and in Monteiro pigs. A D-loop fragment of approximately 330 bp was amplified by PCR. Sequencing of DNA amplicons identified haplotypes previously described as European and Asian types. Monteiro pigs and wild pigs had European haplotypes and domestic pigs had both European and Asian haplotypes.


Subject(s)
Animals , DNA, Mitochondrial/analysis , Genetic Variation , Sus scrofa/genetics , Animals, Wild , Base Sequence , Haplotypes , Molecular Sequence Data , Polymerase Chain Reaction , Polymorphism, Genetic/genetics , Sus scrofa/classification
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